Table 2.
The ventral vegetal lineage expresses a retinal fate only when vegetal factors, anti-neural factors and posterior-axis promoting factors are inhibited
| Injected mRNAs or morpholinos |
ISH |
Fate Mapping |
||
|---|---|---|---|---|
| ectopic expression (% embryos) |
clone populates (% embryos) |
|||
| sox3 (n) | rx1 (n) | neural (n) | retina (n) | |
| β-gal | 0 (23) | 0 (31) | - | - |
| gfp | - | - | 0 (23) | 0 (23) |
| VegTMO | 0 (32) | 0 (32) | 30 (27) | 0 (27) |
| dnVg1 | 0 (24) | 0 (26) | 32 (25) | 0 (25) |
| VegTMO/dnVg1 | 0 (32) | 0 (34) | 33 (18) | 0 (18) |
| cer-L | 38 (32) | 33 (48) | 80 (30) | 27 (30) |
| nog/tBR/dnWnt | 41 (32) | 32 (38) | 79 (19) | 0 (19) |
| cer-S | - | 0 (37) | - | 0 (31) |
| cm-Xnr2 | - | 0 (46) | - | 0 (42) |
| cer-S/cm-Xnr2 | - | 0 (38) | - | 0 (34) |
| cm-derriere | - | 0 (30) | - | 0 (27) |
| VegTMO/cer-S | - | 0 (26) | - | 0 (34) |
| VegTMO/cm-Xnr2 | - | 0 (38) | - | 0 (28) |
| VegTMO/cm-derriere | - | 0 (34) | - | 0 (22) |
| VegTMO/nog/tBR/dnWnt | 59 (32) | 37 (38) | 50 (29) | 38 (26) |
| VegTMO/tBR | - | 0 (26) | - | 0 (34) |
| VegTMO/dnWnt | - | 0 (31) | - | 0 (27) |
| VegTMO/dnVg1/nog/tBR/dnWnt | 100 (30) | 100 (26) | 100 (6) | 45 (29) |
Legend: Reagents were injected into one V2.1.1 blastomere with appropriate lineage tracer (β-Gal for ISH; GFP for fate mapping). Embryos were fixed at neural plate stages for ISH and stages 37/38 for fate mapping.