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. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: Dev Biol. 2007 Feb 7;305(1):103–119. doi: 10.1016/j.ydbio.2007.01.040

Table 2.

The ventral vegetal lineage expresses a retinal fate only when vegetal factors, anti-neural factors and posterior-axis promoting factors are inhibited

Injected mRNAs or
morpholinos
ISH
Fate Mapping
ectopic expression
(% embryos)
clone populates
(% embryos)  
sox3 (n) rx1 (n) neural (n) retina (n)
β-gal 0 (23) 0 (31) -   -  
gfp -   -   0 (23) 0 (23)
VegTMO 0 (32) 0 (32) 30 (27) 0 (27)
dnVg1 0 (24) 0 (26) 32 (25) 0 (25)
VegTMO/dnVg1 0 (32) 0 (34) 33 (18) 0 (18)
cer-L 38 (32) 33 (48) 80 (30) 27 (30)
nog/tBR/dnWnt 41 (32) 32 (38) 79 (19) 0 (19)
cer-S -   0 (37) -   0 (31)
cm-Xnr2 -   0 (46) -   0 (42)
cer-S/cm-Xnr2 -   0 (38) -   0 (34)
cm-derriere -   0 (30) -   0 (27)
VegTMO/cer-S -   0 (26) -   0 (34)
VegTMO/cm-Xnr2 -   0 (38) -   0 (28)
VegTMO/cm-derriere -   0 (34) -   0 (22)
VegTMO/nog/tBR/dnWnt 59 (32) 37 (38) 50 (29) 38 (26)
VegTMO/tBR -   0 (26) -   0 (34)
VegTMO/dnWnt -   0 (31) -   0 (27)
VegTMO/dnVg1/nog/tBR/dnWnt 100 (30)   100 (26)   100 (6)   45 (29)

Legend: Reagents were injected into one V2.1.1 blastomere with appropriate lineage tracer (β-Gal for ISH; GFP for fate mapping). Embryos were fixed at neural plate stages for ISH and stages 37/38 for fate mapping.