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. 2003 Dec;163(6):2565–2574. doi: 10.1016/s0002-9440(10)63611-5

Figure 5.

Figure 5.

PDGF (A-chain and B-chain) expression following addition of 25 mmol/L d-glucose. Growth-arrested HK-2 cells were stimulated with 25 mmol/L d-glucose under serum-free conditions and total mRNA isolated at the time points indicated (A). In control experiments, cells were exposed to 5 mmol/L d-glucose alone. For PDGF-A chain and PDGF-B chain, PCR amplification was performed for 30 cycles. PCR amplification for GAPDH was performed for 25 cycles. Ethidium bromide-stained PCR products were separated on a 3% agarose gel. One representative experiment of three individual experiments is shown. B: The effect of d-glucose on PDGF-A protein expression was determined by collection of supernatant samples between the time points indicated, following addition of either 25 mmol/L d-glucose or 5 mmol/L d-glucose under serum-free conditions and quantification of PDGF by ELISA. Data represents mean ± SEM of four individual experiments. At each time point there was no significant difference in PDGF concentration between the supernatants collected from cells exposed to either 5 mmol/L or 25 mmol/L d-glucose.