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. 2003 Dec;163(6):2619–2634. doi: 10.1016/S0002-9440(10)63616-4

Figure 9.

Figure 9.

Cav-3 mutants impede the recruitment of PFK-M to the plasma membrane. Cos-7 cells were co-transfected with the cDNA encoding V5-tagged PFK-M, in combination with each Cav-3 mutant. Thirty-six hours after transfection, the cells were formaldehyde-fixed and doubly immunostained with a monoclonal antibody directed against the V5 epitope and with a polyclonal antibody directed against Cav-3. Note that expression of Cav-3 mutants, namely Cav-3 P104L (A), Cav-3 ΔTFT (B), and Cav-3 R26Q (C) impedes the recruitment of PFK-M to the cell membrane. N, Nucleus. Scale bars, 10 μm.