Table 1.
Microarray Identification of Genes Activated or Repressed Downstream of EWS-WT1(-KTS)
| Gene symbol | Accession number | B12 | C9 | D9 | Average |
|---|---|---|---|---|---|
| RGS5 | NM_003617 | 4.8069 | 4.5771 | 4.9195 | 4.7678 |
| DKFZp434B227 | NM_032263 | 4.3451 | 4.6415 | 4.9425 | 4.6430 |
| DES (desmin) | NM_001927 | 4.8052 | 3.3748 | 5.1433 | 4.4411 |
| APOA1 | NM_000039 | 3.4595 | 5.0025 | 3.7444 | 4.0688 |
| CHI3L1 | NM_001276 | 3.4901 | 4.4559 | 3.8189 | 3.9216 |
| WT1 | NM_024424 | 3.9531 | 3.8653 | 3.8197 | 3.8794 |
| NK4 | NM_004221 | 2.7691 | 4.3032 | 4.1529 | 3.7417 |
| APOL2 | NM_030882 | 3.5625 | 4.3115 | 2.9113 | 3.5951 |
| FLJ20245 | NM_017723 | 3.4295 | 3.6399 | 2.9387 | 3.3360 |
| ADM (adrenomedullin) | NM_001124 | 3.2513 | 4.1579 | 2.4834 | 3.2975 |
| LOC135562 | XM_069429 | 3.0812 | 4.5899 | 2.0095 | 3.2269 |
| BAIAP3 | NM_003933 | 2.6486 | 4.0179 | 2.6717 | 3.1127 |
| BIK | NM_001197 | 2.3391 | 3.4097 | 3.3323 | 3.0270 |
| LOC90189 | XM_029748 | 2.9116 | 2.7470 | 2.8376 | 2.8321 |
| FLJ22671 | NM_024861 | 2.4854 | 2.7474 | 3.1525 | 2.7951 |
| KLK6 | NM_002774 | 2.8500 | 2.5292 | 2.7768 | 2.7187 |
| LCK | NM_005356 | 1.7054 | 2.2152 | 4.1992 | 2.7066 |
| IL8 | NM_000584 | 1.9838 | 3.7999 | 2.2517 | 2.6785 |
| DRP2 | NM_001939 | 1.9726 | 2.7053 | 3.2519 | 2.6433 |
| KREMEN2 | NM_024507 | 2.3643 | 2.9575 | 2.3405 | 2.5541 |
| TNFRSF21 | NM_014452 | 2.3449 | 2.9221 | 2.2719 | 2.5130 |
| SSTR3 | NM_001051 | 2.7267 | 2.5529 | 2.1279 | 2.4692 |
| FLJ20154 | NM_017787 | 1.8621 | 2.3683 | 2.9680 | 2.3995 |
| NFATC1 | NM_006162 | 2.3952 | 2.8337 | 1.9534 | 2.3941 |
| GAGE7 | NM_021123 | 3.0143 | 2.0046 | 2.1603 | 2.3931 |
| SNX9 | NM_016224 | 2.3430 | 2.3643 | 2.2830 | 2.3301 |
| TM4SF2 (Talla-1) | NM_004615 | 1.8004 | 2.4611 | 2.6285 | 2.2967 |
| LOC139728 | XM_060051 | 2.2622 | 2.3246 | 2.1933 | 2.2600 |
| TRIM29 | NM_012101 | 2.0576 | 2.8573 | 1.8001 | 2.2383 |
| SERPINB2 | NM_002575 | 2.1313 | 2.2954 | 2.2759 | 2.2342 |
| RELB | NM_006509 | 1.9808 | 2.8510 | 1.8384 | 2.2234 |
| FLJ23058 | NM_024696 | 2.1799 | 1.9497 | 2.5029 | 2.2108 |
| IL2RB (IL2 receptor β) | NM_000878 | 2.4299 | 2.5900 | 1.5083 | 2.1761 |
| HSPC022 | NM_014029 | 2.2705 | 2.4120 | 1.8273 | 2.1699 |
| MSLN | NM_005823 | 1.9397 | 2.2261 | 2.2971 | 2.1543 |
| OPRL1 | NM_000913 | 1.9394 | 2.7325 | 1.5796 | 2.0838 |
| MAGP2 | NM_003480 | 2.3341 | 2.0496 | 1.6075 | 1.9971 |
| NEDD4L | NM_015277 | 1.7847 | 2.4666 | 1.7302 | 1.9938 |
| SLC2A3 | NM_006931 | 1.6088 | 2.2489 | 2.1073 | 1.9883 |
| BGN | NM_001711 | 1.5921 | 1.9808 | 2.3423 | 1.9717 |
| COL6A3 | NM_004369 | 1.8971 | 2.3618 | 1.5310 | 1.9300 |
| S100A4 | NM_002961 | 1.8033 | 2.2082 | 1.6691 | 1.8935 |
| KRT17 | NM_000422 | 1.6139 | 2.2038 | 1.6792 | 1.8323 |
| MS4A6A | NM_022349 | 1.7096 | 2.0112 | 1.5267 | 1.7492 |
| C18orf1 | NM_004338 | −1.5796 | −1.5385 | −1.6386 | −1.5856 |
| H1F2 | NM_005319 | −1.9450 | −1.7604 | −1.8163 | −1.8406 |
| OSBPL10 | NM_017784 | −1.6272 | −2.2958 | −1.6578 | −1.8603 |
| FXYD6 | NM_022003 | −1.5180 | −2.1517 | −1.9351 | −1.8683 |
| HNOEL-iso | NM_020190 | −1.8806 | −2.7950 | −1.5057 | −2.0604 |
| P311 | NM_004772 | −2.0767 | −2.2441 | −1.9064 | −2.0757 |
| NECL1 | NM_021189 | −1.5862 | −3.3417 | −1.9093 | −2.2791 |
| FLJ20716 | NM_017938 | −2.0441 | −2.7866 | −2.2385 | −2.3564 |
| H2AFL | NM_003512 | −2.4452 | −2.2226 | −2.4156 | −2.3611 |
| BRAK | NM_004887 | −2.2034 | −2.7810 | −2.4533 | −2.4792 |
| CRTL1 | NM_001884 | −1.9105 | −3.5013 | −2.0543 | −2.4887 |
| SERPINF1 | NM_002615 | −1.8785 | −3.0730 | −2.6775 | −2.5430 |
| MMP2 | NM_004530 | −2.4785 | −2.8093 | −2.4158 | −2.5679 |
| LOC51316 | NM_016619 | −2.7566 | −3.4258 | −2.2395 | −2.8073 |
| SRCRB4D | NM_080744 | −3.4141 | −3.1876 | −3.4979 | −3.3665 |
| MVD | NM_002461 | −3.1784 | −3.7718 | −3.9392 | −3.6298 |
Expression levels (log2 values) of 9,600 human genes in the three EWS-WT1(-KTS)-expressing U2OS clones (B12, C9, and D9) relative to a mixture of control mock-transfected clones are calculated as expression ratios processed from the raw data of two individual experiments labeled reciprocally with two different fluors. Genes listed in the table show more or less than 1.5 of expression level (log2 values) in all three clones. A more detailed description of each gene is available on the National Center for Biotechnology Information website (http://www.ncbi.nlm.nih.gov/).