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. 2003 Dec;163(6):2347–2370. doi: 10.1016/S0002-9440(10)63591-2

Figure 9.

Figure 9.

Immunofluorescence staining of inguinal lymph nodes of EBOV-Zaire-infected cynomolgus monkeys for cell markers and EBOV. A: Double labeling for a macrophage marker (green) and EBOV antigens (red). Areas positive for both macrophage markers and EBOV antigens are stained gold as shown in the single EBOV-positive macrophage at day 3. B and D: Double labeling for a dendritic cell marker (DC-SIGN) (red) and EBOV antigens (green) showing a circulating EBOV-positive dendritic cell at day 3 (B) and large numbers of EBOV-positive dendritic cells (orange/gold) at day 5 (D). C: Double labeling for a macrophage marker (red) and EBOV antigens (green) demonstrating EBOV-positive macrophages (orange/gold) at day 5. E: Double labeling for a dendritic cell marker (DC-SIGN) (green) and EBOV antigens (red) demonstrating EBOV-positive dendritic cell (orange/gold) at day 4. Also, note EBOV-positive cell (red) with macrophage-like morphology and EBOV-negative dendritic cells (green) in this field. Inset: high-power view of the EBOV-positive dendritic cell in E by confocal microscopy. The nuclei were stained with DAPI (blue) in all panels. Original magnifications: ×40 (A and D); ×60 (B, C, E); ×300 (inset).

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