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. 2003 Dec;163(6):2459–2476. doi: 10.1016/s0002-9440(10)63601-2

Figure 7.

Figure 7.

TGF-βRI immunostaining of wounded E16 and E19 rat skin. A: E16 skin, 24 hours post-injury; magnification, ×100. Up-regulated TGF-βRI expression is evident in both epidermal and dermal layers after injury. Non-wounded E17 [ie, E16 + 24 hours] control skin is also shown (inset, A, yellow rectangle). B: E16 skin, 24 hours post-injury; magnification, ×400. Higher magnification view of wounded epidermis demonstrating strong TGF-βRI staining in outer, basal and migrating (black open arrow) epidermal layers. C: E16 skin, 24 hours post-injury; magnification, ×400. Increased TGF-βRI staining from minimal to moderate levels is observed in dermal fibroblasts (blue open arrows) as well as in inflammatory cells (red open arrows) after injury. D: E16 skin, 36 hours post-injury; magnification, ×400. Except for some minimal TGF-βRI up-regulation in inflammatory cells, no epidermal or dermal components demonstrate any TGF-βRI expression above control non-wounded skin. Note the complete absence of TGF-βRI staining in fibroblasts (blue open arrows). Non-wounded E17.5 [ie, E16 + 36 hours] control skin is also shown (inset, D, yellow rectangle). E: E19 skin, 24 hours post-injury; magnification, ×100. Epidermal and dermal TGF-βRI localization is not increased relative to non-wounded control skin. Non-wounded E20 [ie, E19 + 24 hours] control skin is also shown (inset, E, yellow rectangle). F: E19 skin, 24 hours post-injury; magnification, ×400. Moderate epidermal staining is observed in the migrating (black open arrow) as well as the outer and basal layers. G: E19 skin, 24 hours post-injury; magnification, ×400. Inflammatory cells (red open arrows) show moderate TGF-βRI staining whereas fibroblasts (blue open arrows) and dermal ECM demonstrate no staining. H: E19 skin, 36 hours post-injury; magnification, ×400. Wounded E19 skin demonstrate up-regulated TGF-βRI expression in dermal fibroblasts (blue open arrows), inflammatory cells (red open arrows), and ECM. Non-wounded, E20.5 [ie, E19 + 36 hours] control skin (inset, H, yellow rectangle) exhibit minimal dermal TGF-βRI staining. Bars: A and E, 200 μm; B–D, F–H, 50 μm. I. RT-PCR of TGF-βRI. Please refer to the Figure 4Ic ▶ legend for an explanation of the bar graphs and representative Southern blot data.