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. 2003 Dec;163(6):2459–2476. doi: 10.1016/s0002-9440(10)63601-2

Table 1.

Description of Primary Antibodies Used for Indirect Immunostaining

Antibody Initial concentration Origin Epitope recognition Final concentration (dilution in PBS)
TGF-β1 200 μg/ml Rabbit polyclonal IgG Extreme carboxy terminus of TGF-β1 (region of mature peptide) 0.002 μg/μl (1:100)
TGF-β2 200 μg/ml Rabbit polyclonal IgG Extreme carboxy terminus of TGF-β2 (region of mature peptide) 0.002 μg/μl (1:100)
TGF-β3 200 μg/ml Rabbit polyclonal IgG Extreme carboxy terminus of TGF-β3 (region of mature peptide) 0.0027 μg/μl (1:75)
TGF-β RI (R-20) 200 μg/ml Rabbit polyclonal IgG Carboxy terminus of TGF-β RI (region of mature peptide) 0.002 μg/μl (1:100)
TGF-β RII (L-21) 200 μg/ml Rabbit polyclonal IgG Amino terminus of TGF-β RII (region of signal and mature peptide) 0.002 μg/μl (1:100)
TGF-β RII (H-567) 200 μg/ml Rabbit polyclonal IgG Full length TGF-β RII (entire peptide) 0.002 μg/μl (1:100)
TGF-β RIII (C-20) 200 μg/ml Goat polyclonal IgG Carboxy terminus of TGF-β RIII (region of mature peptide) 0.002 μg/μl (1:100)