Table 1.
Description of Primary Antibodies Used for Indirect Immunostaining
| Antibody | Initial concentration | Origin | Epitope recognition | Final concentration (dilution in PBS) |
|---|---|---|---|---|
| TGF-β1 | 200 μg/ml | Rabbit polyclonal IgG | Extreme carboxy terminus of TGF-β1 (region of mature peptide) | 0.002 μg/μl (1:100) |
| TGF-β2 | 200 μg/ml | Rabbit polyclonal IgG | Extreme carboxy terminus of TGF-β2 (region of mature peptide) | 0.002 μg/μl (1:100) |
| TGF-β3 | 200 μg/ml | Rabbit polyclonal IgG | Extreme carboxy terminus of TGF-β3 (region of mature peptide) | 0.0027 μg/μl (1:75) |
| TGF-β RI (R-20) | 200 μg/ml | Rabbit polyclonal IgG | Carboxy terminus of TGF-β RI (region of mature peptide) | 0.002 μg/μl (1:100) |
| TGF-β RII (L-21) | 200 μg/ml | Rabbit polyclonal IgG | Amino terminus of TGF-β RII (region of signal and mature peptide) | 0.002 μg/μl (1:100) |
| TGF-β RII (H-567) | 200 μg/ml | Rabbit polyclonal IgG | Full length TGF-β RII (entire peptide) | 0.002 μg/μl (1:100) |
| TGF-β RIII (C-20) | 200 μg/ml | Goat polyclonal IgG | Carboxy terminus of TGF-β RIII (region of mature peptide) | 0.002 μg/μl (1:100) |