Representative results of loss of heterozygosity analysis. DNA was prepared from normal tissue and different components of malignant mixed germ cell tumor, amplified by polymerase chain reaction using polymorphic markers D1S508, D2S156, D9S162, D11S903, D12S1051, and D18D46, and separated by gel electrophoresis. Arrows, allelic bands; N, normal tissue (control); MT, mature teratoma; IMT, immature teratoma; EC, embryonal carcinoma; YST, yolk sac tumor; CC choriocarcinoma, SE, seminoma.