Figure 2.

Properties of tumor-derived pcDNA3- and IL-1α-overexpressing clones. A: NF-κB DNA-binding activity in pcDNA3- and IL-1α-overexpressing cells isolated from tumors and grown in culture (lanes 1 to 4). CM from the same cells was tested for their ability to induce NF-κB in HLF-1 cells (lanes 5 to 9). NF-κB DNA binding was measured as in Figure 1A ▶ . B: Neutralizing antibody against IL-1α blocks NF-κB activation by Td-IL-1α1 CM. CM was treated with the indicated neutralizing antibodies (3 μg/ml) for 1 hour at room temperature. Cells were treated with CM for 1 hour.