Figure 3.

MMP activation and growth of Td-pcDNA3-1 and Td-IL-1α3 cells in the presence of HLF-1 cells. A: Gelatin zymography using CM from either cancer cells alone (lanes 1 to 7), HLF-1 (lane 8), or cancer cells in combination with HLF-1 cells. B: Rate of tumor growth. Cancer cells (2 × 10⁶) with or without HLF-1 cells (2 × 10⁵) were injected into the mammary pads of nude mice with estrogen pellet implants. Tumor growth was measured once a week. C: Analysis of lung DNA for metastasis of cancer cells. DNA from lungs was isolated and subjected to PCR with human β-globin-specific primers. As a control, PCR was also performed with primers that amplify mouse requiem genomic DNA. 77 Southern blotting using an internal primer identified PCR products.