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. 2003 Dec;163(6):2531–2541. doi: 10.1016/s0002-9440(10)63608-5

Figure 4.

Figure 4.

The effect of IL-1α expression in cancer cells on body weight and mouse leptin transcripts. A: Weight of animals implanted with Td-pcDNA3-1 (square) or Td-IL-1α3 (triangle) (n = ∼30). Weight of animals without any tumor is also shown (circle). P = <0.0001 nontumor versus Td-IL-1α3; P = 0.0003 Td-pcDNA3-1 versus Td-IL-1α3; P = 0.0839 nontumor versus Td-pcDNA3-1. B: Skin phenotype of Td-pcDNA3 and Td-IL-1α tumor-bearing animals. Cross sections of dorsal skin show atrophy in Td-IL-1α tumor-bearing animals compared to Td-pcDNA3 tumor-bearing animals. SM, skeletal muscle; HF, hair follicle; SG sebaceous glands; ED, epidermis. C: Human LMF and mouse leptin-specific transcript levels in tumor samples. Total RNA from tumors was subjected to RT-PCR (35 cycles) using primers that specifically amplify mouse leptin RNA. Primers corresponding to human LMF were used to amplify LMF (35 cycles). Quality of RNA as well as cDNA synthesis was verified by PCR amplification of the housekeeping gene GAPDH (18 cycles). Southern blotting of PCR products with an internal primer as a probe and autoradiography identified PCR products. Because of limited amplification, PCR products were not visible by ethidium bromide staining.