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The effect of IL-1α expression in cancer cells on muscle protein status and serum cytokine profile. A: Differences in the level of MyoD, ubiquitinated protein, and α-tubulin in leg muscle. Muscle extracts were prepared using RIPA buffer and 50 μg of protein was subjected to Western blotting with indicated antibodies. B: Profile of various cytokines in serum of animals implanted with different cell types.
