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. 2000 Dec 19;97(26):14364–14369. doi: 10.1073/pnas.97.26.14364

Figure 2.

Figure 2

(A) Expression of AGS3 mRNA. Analysis of a rat multiple tissue Northern blot with an AGS3 probe shows a 2.3-kb mRNA in heart (lane 1) and a 3.5-kb mRNA in brain (lane 2). No mRNAs were detected in spleen (lane 3), liver (lane 4), lung (lane 5), skeletal muscle (lane 6), kidney (lane 7), or testis (lane 8) under these conditions. (B) Expression of AGS3 protein. Analysis of a rat multiple tissue Western blot (75 μg protein/lane) with affinity-purified AGS3 IgG (8.5 μg/ml in TBS, 0.1% Tween 20, 5% FCS) showing that AGS3 protein is widely expressed. A 75-kDa protein is detected in brain (lane 2) and testis (lane 6). Liver (lane 1), kidney (lane 4), heart (lane 8), and pancreas (lane 9) showed a single protein band at 53, 42, 26, and 35 kDa, respectively. No AGS3 immunoreactivity was detected in lung (lane 3), spleen (lane 5), and ovary (lane 7) under these conditions.