Skip to main content
. 2000 Dec 19;97(26):14364–14369. doi: 10.1073/pnas.97.26.14364

Figure 3.

Figure 3

Distribution of AGS3 protein in membrane and cytosolic fractions of PC-12 cells. A postnuclear supernatant (PNS) was centrifuged at 100,000 × g to yield particulate (P) and cytosolic (C) fractions. These fractions (normalized by volume) were immunoblotted with affinity-purified AGS3 IgG and detected by ECL, and the amount found in each fraction was quantified by densitometry. The majority of AGS3 (>95%) is found in the cytosolic fraction, but a small amount (5%) is found in the particulate fraction.