Table 1.

AGS3 interacts with Gα_i subfamily members

Bait	β-gal	Prey
Gαi1	+++	AGS3361–650
Gαi2	++	AGS3361–650
Gαi3	+++	AGS3361–650
Gαi3	++++	AGS3361–590
Gαo	++	AGS3361–650
Gαz	−	AGS3361–650
Gαs	−	AGS3361–650
Gαq	−	AGS3361–650
Gα12	−	AGS3361–650
Gpa1	−	AGS3361–650

The β-gal filter assay was performed on (Leu⁻, Trp⁻) plates, and intensity of color was scored after 8 h. −, no color; ++, moderate color; +++, strong color; ++++, very strong color. Yeast cotransformed with void bait and prey vectors were taken as background (none detected after 20 h). Baits were constructed in pGBT9, and AGS3 preys were in pACT2. Three independent experiments were performed with the same results.