Table 1.
Gene Expression for Mouse NFkB Signaling at 6 Hours after A. fumigatus Conidia Challenge in Mice Subjected to Sham or CLP Surgery
| Groups | Genes | Sham + asp | CLP + asp | |||||
|---|---|---|---|---|---|---|---|---|
| up | down | |||||||
| 1° | 2° | 3° | 4° | 1° | 2° | 3° | ||
| CREBP1/ATF-2 | 2.2 | 3.2 | 2.5 | −3.9 | 0 | |||
| Elk-3 | 2.9 | 3.7 | −2.7 | 0 | 0 | |||
| Signal transduction kinase | IKK-γ | 3.1 | 12.0 | 3.8 | ||||
| JNK1 | 6.7 | 3.0 | 2.1 | |||||
| MKK4 (JNKK1) | 3.7 | 2.4 | 0 | −2.7 | ||||
| IkBα | 4.7 | 2.6 | 3.0 | 0 | 0 | |||
| Rel/NFkB/IkB family | NFkBIB | 4.0 | 3.2 | 3.0 | 3.0 | −2.2 | 0 | |
| NFkB1 | 5.4 | 2.1 | ||||||
| NFkB2 | 3.9 | 2.0 | −4.2 | −5.3 | ||||
| c-rel | 9.1 | 6.8 | 2.6 | 3.7 | 0 | 0 | ||
| Adaptor proteins | IRAK2 | 2.1 | 2.4 | |||||
| MyD88 | 2.1 | 2.4 | 2.0 | −6.4 | −2.9 | |||
| Cytokine/cytokine receptor | IL-1β | 2.9 | 10.3 | 11.5 | 6.4 | −2.6 | 0 | −3.6 |
| IL-1R1 | 3.3 | 2.6 | 0 | 0 | 0 | |||
| Others | L-selectin | 5.2 | 2.9 | 0 | −2.5 | |||
Mice were sacrificed 6 hours after A. fumigatus injection and the lung was harvested and immediately snap-frozen to subsequent mRNA purification and gene expression analysis by Gene Array. The results are expressed as fold increase or decrease relative to the control group, and values of each group were normalized with three or four housekeeping genes present on the membrane. The first column contains genes expressed in the sham + Asp challenged mice compared with sham + saline group; the second column contains genes expressed in the CLP + Asp compared to sham + Asp group. The O value means that the gene was not expressed when compared to sham + Asp group which showed expression of this gene. The experiment was repeated four times for sham + Asp compared to sham + saline and three times for CLP + Asp compared to sham + Asp. Each membrane was hybridized with mRNA purified from lung obtained from three to four mice per group.