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CRIM-Specific Activity of Mutant mGCases Expressed in Insect Cells
| Source | Relative GCase activity |
|---|---|
| WT | 100% |
| N370S | 15.4 ± 5.1% |
| V394L | 12.4 ± 3.4% |
| D409H | 12.9 ± 3.5% |
| D409V | 21.6 ± 4.8% |
GCase activity assays were conducted with mGCases harvested from media of Sf21 insect cells infected with the appropriate recombinant baculovirus. CRIM amounts were determined by densitometric scans of immunoblots. Values ± SD were from three determinations.
