Table 2.

Oligonucleotide Primers Used for RT-PCR

Designation	Sequences	Conditions (cycle number)	Message size
18S	+: 5′ TCAAGAACGAAAGTCGGAGGTTCG 3′	1 minute 94°C	475 bp
	−: 5′ TTATTGCTCAATCTCGGGTGGCTG 3′	1 minute 62°C
		1 minute 72°C
		(28 cycles)
OPG	+: 5′ GCTAACCTCACCTTCGAG 3′	30 seconds 94°C	324 bp
		30 seconds 55°C
	−: 5′ TGATTGGACCTGGTTACC 3′	30 seconds 72°C
		(40 cycles)
RANKL	+: 5′ GCCAGTGGGAGATGTTAG 3′	30 seconds 94°C	486 bp
		30 seconds 55°C
	−: 5′ TTAGCTGCAAGTTTTCCC 3′	30 seconds 72°C
		(40 cycles)

Primers are represented in a 5′ to 3′ orientation, with that for the coding strand (+) and the non-coding strand (−). The product size generated by reverse transcription and PCR amplification of the authentic mRNA is indicated, together with semi-quantitative PCR conditions.