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. 2003 Nov;163(5):1997–2008. doi: 10.1016/s0002-9440(10)63558-4

Figure 6.

Figure 6.

NMDA-induced retinal neurotoxicity is prevented by CBD. Rats were given an intravenous injection of CBD suspension immediately before the intravitreal injection of NMDA. Effect of systemic CBD (2 mg/kg) on the NMDA (80 nmol/eye)-induced formation of nitrotyrosine and retinal apoptosis was determined at 18 hours after NMDA injection. OCT-frozen eye sections (8 μm) perpendicular to the retinal surface were analyzed as described before. A: Effect of CBD on the NMDA-induced relative optical density of nitrotyrosine in retinal cryosections determined by immunohistochemistry. All values represent the mean ± SE of a total of four animals for each group in two experiments. *, Significant at P < 0.05. B: Representative retinal distribution of NMDA-induced TUNEL-positive cells in retinal sections after injection of CBD. C: Quantitative analysis of the effect of CBD on the NMDA-induced apoptosis in the retinal cryosections determined by TUNEL assay. Values represent the mean ± SE of a total of four animals for each dose in two experiments. *, Significant at P < 0.01.