Table 1.
Increased Neutrophilia and Decreased Eosinophilia in LPS-Expressed CRAg-Sensitized Mice
| Group | n | Total serum IgE (μg/ml)* | Lung (Rt. lobe) | Peribronchial eosinophils (cells/20 HPF) | Bronchoalveolar lavage | ||||
|---|---|---|---|---|---|---|---|---|---|
| Leukocytes (×106) | CD4+ (×105) | Total leukocytes (cells/HPF) | PMN (%) | Eosinophils (%) | Lymphs (%) | ||||
| Exp. 1 | |||||||||
| No LPS | 4 | 5.4 ± 1.4† | 6.7 ± 0.7 | 4.9 ± 0.6 | N.D.‡ | 15.5 ± 2.5 | 48.8 ± 4.8 | 4.3 ± 1.5 | 2.4 ± 0.4 |
| LPS, 0.3 μg | 5 | 6.6 ± 1.9 | 9.0 ± 0.8§ | 6.2 ± 0.7 | N.D. | 21.1 ± 2.0§ | 70.2 ± 6.0§ | 6.3 ± 2.4 | 1.4 ± 0.6 |
| LPS, 15 μg | 3 | 6.4 ± 1.7 | 20.9 ± 1.0§ | 10.6 ± 0.5§ | N.D. | 47.1 ± 3.1§ | 81.2 ± 0.4§ | 0.0 ± 0.0§ | 0.9 ± 0.1§ |
| Exp. 2 | |||||||||
| No LPS | 4 | 4.8 ± 1.2 | N.D. | N.D. | 139 ± 59 | 14.3 ± 2.4 | 44.9 ± 10.6 | 9.2 ± 5.9 | 2.2 ± 0.3 |
| LPS, 0.6 μg | 4 | 3.6 ± 1.2 | N.D. | N.D. | 77 ± 20 | 27.1 ± 2.8§ | 82.3 ± 2.4§ | 1.2 ± 0.4§ | 0.9 ± 0.3§ |
| LPS, 3 μg | 4 | 4.5 ± 2.3 | N.D. | N.D. | 62 ± 24 | 25.8 ± 2.3§ | 82.1 ± 3.6§ | 3.1 ± 1.4 | 1.8 ± 0.5 |
| LPS, 15 μg | 3 | 4.2 ± 1.5 | N.D. | N.D. | 18 ± 6§ | 62.3 ± 5.4§ | 93.1 ± 2.8§ | 0.1 ± 0.1§ | 0.8 ± 0.5§ |
*Serum IgE level in non-CRAg-sensitized mice was 1.0 ± 0.4 μg/ml of serum.
†Data are mean ± standard error.
‡N.D. = not determined.
§Statistically significant difference (P < 0.05) from no LPS control.