Table 1.
Cellular and Humoral Immunity Four Weeks after Immunization with mBSA/CEA
| Cellular immunity (SI) | Humoral immunity (titer) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 50 | 25 | 12 | 6 | 3 | IgG | IgG1 | IgG2a | IgG2b | IgG3 | |
| WT | 3 (1) | 5 (1) | 6 (2) | 7 (1) | 5 (1) | 13 (2) | 11 (1) | 12 (2) | 11 (1) | 10 (1) |
| FcγRI/III−/− | 5 (2) | 7 (1) | 8 (1) | 8 (0) | 5 (0) | 14 (2) | 13 (1) | 13 (1) | 11 (1) | 11 (0) |
| WT | 1 (1) | 2 (1) | 5 (0) | 6 (1) | 4 (1) | 17 (1) | 14 (2) | 13 (1) | 14 (0) | 10 (1) |
| FcγRI/II/III−/− | 1 (0) | 4 (2) | 6 (1) | 7 (1) | 5 (0) | 18 (1) | 16 (2) | 15 (1) | 14 (1) | 11 (2) |
Cellular immunity was measured by spleen lymphocyte proliferation and expressed as stimulation index (ratio of T cells stimulated with various concentrations of mBSA (50, 25, 12, 6, 3 μg/ml) and T cells not stimulated. Values represent the mean ± SD of three groups of two mice each. Humoral immunity was measured as antibody production against mBSA and various isotypes were determined using ELISA. The sera of six mice were individually tested. Values represent the mean titer ± SD. Note that IgG1, IgG2a, and IgG3 levels were elevated (2–4 times) whereas IgG2b was not elevated in both FcγRI/III−/− and FcγRI/II/III−/− when compared to controls.