Figure 1.

Aortas from atherosclerotic ApoE mice injected with 10¹¹ phage either expressing CAPGPDSKSC or not (control phage). Binding of phage to aorta was visualized with biotinylated anti-phage antibody and streptavidin-linked peroxidase activation of DAB. A: The aorta of an atherosclerotic ApoE knockout mouse injected with CAPGPSKSC phage. Phage binding to the lesions is readily visualized (red-brown). B: The aorta from an atherosclerotic ApoE knockout mouse in which lesions are clearly evident. This mouse was infused with control phage and there is no evident association of phage with the lesions. C: The normal aorta from a BALB/c mouse infused with the CAPGPSKSC phage. No association of phage with the aortic surface is observed suggesting the CAPGPSKSC target molecule is absent or not present in detectable quantity. D: The aorta of a young ApoE knockout mouse on standard chow diet and without atherosclerotic lesions when infused with CAPGPSKSC phage, no localization was detectable indicating that the CAPGPSKSC target molecule is not expressed simply as a result of ApoE gene inactivation.