Figure 2.

A: An aorta from an atherosclerotic ApoE knockout mouse and infused with the synthetic biotinylated CAPGPSKSC peptide. Peptide binding to the lesions is visualized (red-brown) and replicates the phage binding. B: A histological section of a typical atherosclerotic lesion of the aorta of an atherosclerotic ApoE knockout mouse. Endothelium overlying the atherosclerotic lesion was identified with anti-CD31 antibody and labeled dark brown (arrows). C: An adjacent section. The binding of this peptide is most intense to the endothelium overlying the atherosclerotic lesion (yellow arrows), but negative on endothelial cells not directly overlying lesions (white arrows). D: A positive control with all endothelial cells recognized by an anti-CD31 antibody. E: A negative control and autofluorescence resulted from elastic tissue in the vessel wall. Original magnifications, ×200.