Figure 3.

Image of an atherosclerotic lesion of a human iliac artery segment. The tissue before staining is shown at the left. Binding of biotinylated CAPGPSKSC peptide to the lesion is demonstrated after application to the lumenal surface of the vessel (right). The binding of phage to the lesion was visualized with enzyme-linked streptavidin conversion of DAB substrate to the brownish red product. The association of CAPGPSKSC peptide to the lesion, but not to nonatherosclerotic surface of this and other vessels (not shown), indicates that a human homologue of the target molecule is present. Lesions are outlined.