Figure 6.

Images of phage bound to aortic valves. These aortic valves were from ApoE knockout mice with atherosclerotic lesions. The mice were infused with 10¹¹ pfu of phage carrying the CNQRHQMSC sequence. In A, a H&E-stained section of aortic valve from an atherosclerotic ApoE knockout mouse is shown. Note the aortic valve is thickened by the presence of atherosclerotic lesions. In B, the endothelial cells report red with biotinylated rat anti-mouse CD 31 antibody and the associated phage report green with anti-phage antibody. Co-localization reports yellow. In experiments performed with control phage, no phage association was evident as shown in C. These data indicate that the CNQRHQMSC phage can bind to atherosclerotic lesion-involved endothelial surfaces. Original magnifications, ×100.