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The three synthetic peptides with homology to TIMP-2 bind to endothelial cells in a dose-dependent manner (top). Binding of peptides (0.5 μmol/L) was inhibited by purified TIMP-2 protein (0.05 μmol/L) (bottom), indicating that the peptides mimicking TIMP-2 binding are consistent with a novel binding site for TIMP-2 protein on the surface of these cells. The scrambled control peptide did not bind.
