Table 1.

Nipah Virus Nucleoprotein Gene Specific Primers and RT-PCR Conditions

Primers
5′-CTGCTGCAGTTCAGGAAACATCAG-3′
5′-ACCGGATGTGCTCACAGAACTG-3′
RT-PCR mix
Primers (10 pmol/μl)	5 μl each
dNTP (10 mmol/L)	4 μl
Buffer (5×)	10 μl
DTT (100 mmol/L)	2 μl
RNAse inhibitor (5 U/μl)	1 μl
Titan enzyme mix	1 μl
RNA template	10 to 20 μl
H2O to final volume of 50 μl
RT-PCR conditions
50°C	30 min
94°C	5 min
94°C	1 min × 30 cycles
50°C	1 min × 30 cycles
72°C	2 min × 30 cycles
72°C	10 min
4°C	Hold