Table 2.
RT-PCR Analysis, Nipah Virus Culture, and Pathological Lesions in Infected Hamster Specimens
| Tissue/specimen | No. of animals with pathological lesions/total no. of animals (%) | No. of positive animals/total no. of animals (%) | |||
|---|---|---|---|---|---|
| Intranasal route* | Intraperitoneal route | ||||
| RT-PCR | Virus culture | RT-PCR | Virus culture | ||
| Blood | na† | 0/5 (0%) | nd‡ | 0/2 (0%) | nd |
| Urine | na | 3/3 (100%) | 0/2 (0%) | 2/3 (67%) | 1/1 (100%) |
| Brain | 8/12 (67%) | 4/5 (80%) | 1/5 (20%) | 3/3 (100%) | 2/3 (67%) |
| Spinal cord | 0/7 (0%) | na | na | 3/3 (100%) | 2/2 (100%) |
| Lung | 4/12 (33%) | 4/5 (80%) | 0/5 (0%) | 3/3 (100%) | 2/3 (67%) |
| Kidney | 2/12 (17%) | 4/5 (80%) | 1/5 (20%) | 3/3 (100%) | 2/3 (67%) |
| Spleen | 1/12 (8%) | 5/5 (100%) | 0/5 (0%) | 3/3 (100%) | 1/3 (33%) |
| Liver | 4/12 (33%) | 5/5 (100%) | 0/5 (0%) | 3/3 (100%) | 2/3 (67%) |
| Heart | 3/12 (25%) | 3/5 (60%) | 0/5 (0%) | 3/3 (100%) | 0/3 (0%) |
*The LD50 of animals by intranasal and intraperitoneal routes was respectively 47,000 and 270 plaque forming units for each animal.
†na = tissues not available or not suitable for analysis.
‡nd = tissues available but analysis not done because RT-PCR is negative.