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Immunoblots of GLUT12, GLUT8, and β-tubulin proteins from GT1(+/+) and GT1(+/−) ES cells. This figure identifies for the first time the expression of GLUT12 and GLUT8 in ES cells. Neither isoform was significantly altered in GT1(+/−) cells that have heterozygous inactivation of the GLUT1 gene. GLUT12 was identified at ∼50 kd and GLUT8 at ∼33 kd, consistent with their previously reported sizes in other cell types as described in the text.
