Figure 5.

Anti-sense oligodeoxynucleotides (MOE gapmers at 150 nmol/L) were tested for the ability to reduce CTGF mRNA levels using the bEND cell culture-screening assay. The oligodeoxynucleotide, with the sequence GCC-AGA-AAG-CTC-AAA-CTT-GA, corresponds to nucleotides 987 to 1007 of the human CTGF mRNA, reduced the level of mouse CTGF mRNA in bEND cells by 86%, and was selected as the CTGF anti-sense oligodeoxynucleotide. This is represented by the 12th bar from the left (the oligo with the lowest percent control value). The error bars represent the range from two experiments.