Table 2.
PARC Production after Stimulation of Adherent Monocytes with T-Cell Conditioned Medium
| Inducers* | Sup-T1 | MOLT-4 | |||
|---|---|---|---|---|---|
| Co-stimulus | Cytokine | PARC (ng/ml)† | P value‡ | PARC (ng/ml)† | P value‡ |
| Buffer | none | 0.5 ± 0.2 | — | 0.2 ± 0.1 | — |
| IL-4 | 0.9 ± 0.5 | — | 0.4 ± 0.2 | — | |
| 105 cells | none | 1.2 ± 0.5 | 0.25 | 0.3 ± 0.1 | 0.1 |
| IL-4 | 5.2 ± 1.7 | 0.028 | 1.9 ± 0.4 | 0.008 | |
| CM (1/2) | none | 2.4 ± 1.0 | 0.047 | 0.5 ± 0.2 | 0.063 |
| IL-4 | 6.1 ± 1.9 | 0.016 | 1.7 ± 0.4 | 0.012 | |
| CM (1/20) | none | 0.8 ± 0.3 | 0.59 | 0.2 ± 0.1 | 0.71 |
| IL-4 | 3.4 ± 0.9 | 0.076 | 1.1 ± 0.4 | 0.06 | |
*To stimulate PARC production, adherent monocytes were treated with dilution buffer, 105 Sup-T1 cells or MOLT-4 cells or conditioned medium (CM, diluted 1/2 or 1/20) from these T-cell lines in combination with the cytokine IL-4 (30 ng/ml) or the dilution buffer. Cultured T lymphoblastic cells alone failed to produce significant amounts of PARC, either unstimulated or after addition of IL-4 (not shown).
†After 48 hours, cell supernatants were collected and analyzed for their PARC content by a specific ELISA. Values indicate the mean (± SEM) PARC concentration from 5 (Sup-T1) or 7 (MOLT-4) experiments.
‡P values, calculated by the Mann-Whitney test, indicate differences between co-stimulated and control cultures (adherent monocytes in the presence of the corresponding inducer, ie, dilution buffer or IL-4).