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. 2003 Nov;163(5):2113–2126. doi: 10.1016/S0002-9440(10)63568-7

Figure 5.

Figure 5.

Loss of ER and PR during tumor progression to malignancy in the PyMT model. A to L: IHC of ER-α. Positive cells are stained in brown. A: Hyperplastic lesion from a PyMT mouse at 4 weeks of age. The inset in A is shown in B. Arrows point to some of the ER-positive cells. C: TEB adjacent of the hyperplastic lesion shown in A. The neck of the TEB (inset) is shown in D with higher magnification. Arrows point to ER-α-positive stromal cells and open arrows point to ER-α-positive epithelial cells in the TEB. E: ER-α staining of a primary mammary lesion at adenoma/MIN stage from a PyMT mouse at 8 weeks of age. F: A morphologically normal duct adjacent to the primary lesion shown in E. Arrows point to some of the ER-α-positive cells. G to I: Mosaic staining of ER-α in tumor at the early carcinoma stage. G: A tumor section from a PyMT mouse at 14 weeks of age. The inset H is in an area with high density of ER-α-positive cells and shown in H. Inset I is in an area with low-density staining and shown in I. J: A representative staining of ER-α in primary tumor at the late carcinoma stage (18 weeks of age). K: An area from the same tumor consisting of a limited number of ER-α-positive cells (arrow). The inset in K is shown in L with higher magnification. Arrows point to the positively stained cells. M to P: IHC of PR in mammary tumors from PyMT mice. M: A representative staining of PR in the hyperplastic lesion of a PyMT mouse at 6 weeks of age. Arrows point to some of the positively stained cells. N: Staining in an adenoma/MIN from a PyMT mouse at 8 weeks of age. Arrows point to some PR-positive cells. O: A representative PR staining of tumor at the late carcinoma stage. The tumor is from a PyMT mouse at 15 weeks of age and arrows point to PR-positive cells in a residual duct. P: Mammary section from a PyMT mouse at 8 weeks of age showing a representative staining of PR in morphologically normal mammary duct. Scale bars: 25 μm (A, C, K, M, O, P); 10 μm (B, D, E, F, H, I, J, L, N); 100 μm (G). Original magnifications: ×100 (G); ×1000 (B, D, E, F, H, I, J, L, N); ×400 (A, C, K, M, O, P).