Figure 2.

CaM antagonists induce cleavage of caspases-8, -9, and -3; cleavage of Bid; and release of cytochrome c in IFN-γ-pretreated cells. Fas-low cells were incubated with IFN-γ (250 U/ml) or medium alone for 18 hours followed by an additional 12 hours of incubation with activating Fas Ab (CH11, 250 ng/ml), TMX (10 μmol/L), or TFP (15 μmol/L). To detect caspase and Bid cleavage, whole cell lysates were prepared and immunoblotted for caspases or Bid. For the detection of cytochrome c release, cytosolic extracts were prepared and immunoblotted for cytochrome c.