Figure 1.

Systemic infusion of AngII increases renal CTGF mRNA expression via AT₁. AngII (50 ng/kg/min; n = 8) was infused into rats for 3 days, total cortex RNA was extracted and analyzed by RT-PCR. Some animals were also treated with the AT₁ antagonist Losartan (10 mg/kg/day; n = 8), or the AT₂ antagonist PD123319 (30 mg/kg/day; n = 4), starting 24 hours before AngII infusion. Representative RT-PCR experiment that shows three different animals of each group (bottom) and data of densitometric analysis expressed as arbitrary units of mean ± SEM of four to eight animals of each group analyzed by duplicate (top). * P < 0.05 vs. control; # P < 0.05 vs. AngII infusion.