Figure 9.

TGF-β is involved in AngII-induced CTGF regulation. In mesangial cells, TGF-β (1 ng/ml) increased CTGF mRNA expression (A) and synthesis (B). Cells were treated with AngII (10⁻⁷ mol/L) in the presence of a TGF-β neutralizing antibody (10 μg/ml) for different times. A: Top shows a representative Northern blot; bottom shows values of mean of two experiments. B: Top shows a representative Western blot; bottom shows values of mean ± SEM of four experiments. * P < 0.05 vs. control. # P < 0.05 vs. AngII infusion.