Table 4.
Histological Evaluation of Inflamed Paws in Antibody-Induced Arthritis
| Mouse strain | Treatment | Max score (mean ± SEM) | Histological score |
|---|---|---|---|
| BALB/c | PBS + LPS | 0 | N |
| — | 0 | N | |
| mAbs + LPS | 55.3 ± 4.2 | A3 | |
| DBA/1 | PBS + LPS | 0 | N |
| — | 0 | N | |
| mAbs + LPS | 19.3 ± 5.3 | H1 | |
| B10.RIII | PBS + LPS | 0 | N |
| — | 0 | N | |
| mAbs + LPS | 26.3 ± 4.3 | A2 | |
| QD | PBS + LPS | 0 | N |
| — | 0 | N | |
| mAbs + LPS | 20 ± 2.5 | A2 | |
| QB | PBS + LPS | 0 | N |
| — | 0 | N | |
| mAbs + LPS | 27 ± 4.7 | A3 | |
| B10.Q | PBS + LPS | 0 | N |
| — | 0 | N | |
| mAbs + LPS | 20 ± 2.6 | A1 | |
| C3H.Q | PBS + LPS | 0 | N |
| — | 0 | N | |
| mAbs + LPS | 10 ± 5 | H1 |
Histological scores were evaluated as active score (A) from 1 to 3 and healing score (H) from 1 to 3. Active score 1 (A1) is mild synovitis; score 2 (A2), moderate synovitis with pannus formation; score 3 (A3), severe pannus formation, erosion of bone with more fibrin deposition. Healing score 1 (H1), new cartilage formation; score 2 (H2), cartilage and bone formation, score 3 (H3), cartilage and bone formation with ankylosis. N stands for normal. Three to six paws were examined for each group. Paws collected on day 11 after antibody injection (day 5 after LPS stimulation) were used for histological analysis. —, no treatment and paws were used from age-matched naïve mice.