Figure 2.

Macroscopic view of the passaging procedure. Generation of a HSE with a biopsy obtained from a REBS patient. Primary culture is established by placing a skin biopsy onto fibroblast-populated collagen matrix and culturing for 32 days at the air-liquid interface. After this culture period the collagen matrix was fully covered with keratinocytes originating from the biopsy. To establish a secondary culture, a fragment from the primary HSE is transferred onto fibroblast-populated collagen matrix.