Figure 2.

Growth and morphology of various cells. (A) Various strains were pregrown in YEAU at 30°C and stained with calcofluor. The average length and width of the septated (constant volume) yin6Δ cells are 18.5 ± 1.7 and 5.1 ± 0.2 μm (mean ± SD, n = 20 cells), respectively, compared with 15.4 ± 0.5 and 4.0 ± 0.2 μm (mean ± SD, n = 10 cells) of wild-type cells. We note that the bent cell shape is more pronounced at 30°C than 20°C. (Bar = 5 μm.) (B) Cells after a series of 1:5 dilutions were spotted on YEAU plates, which were incubated at either 30°C or 20°C. At 20°C, the doubling times of yin6Δ and wild-type (WT) cells in rich medium are 8.5 and 5.5 h (and 22.8 and 7.9 h in minimal medium). Strains tested were SP870 (WT), YIN6K (yin6Δ), MOE1L (moe1Δ), and ECP17 (yin6Δ moe1Δ).