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. Author manuscript; available in PMC: 2008 Jun 7.
Published in final edited form as: Regul Pept. 2007 Jan 10;141(1-3):61–72. doi: 10.1016/j.regpep.2006.12.017

Figure 2.

Figure 2

Cell migration (A) and invasion (B) of PTHrP-overexpressing and control LoVo cells. Cells were plated on FluoroBlok inserts in the absence of FBS. To measure invasion, the inserts were overlaid with Matrigel. Cells (0.5 × 106) were loaded with Calcein-AM and plated onto the inserts; FBS (10%) was used as the chemoattractant. Migration and invasion were measured as described in Materials and Methods. Data for each of four independent PTHrP-overexpressing clones (PTHrP1 to PTHrP4), two independent empty vector-transfected clones (V1 and V2; controls), and parental cells (P) are presented. The PTHrP-overexpressing clones secrete between 55-fold and 75-fold more PTHrP than the empty vector-transfected cells. Each bar is the mean ± SEM of three independent experiments. * = Significantly different from the control value (P < 0.001).