Skip to main content
. Author manuscript; available in PMC: 2008 Jan 1.
Published in final edited form as: Dev Cell. 2007 Jan;12(1):31–43. doi: 10.1016/j.devcel.2006.11.001

Figure 5. Anti-Incenp Antibodies Bypass the Requirement of Dasra A in Spindle Microtubule Assembly and Induce Aberrant Achromosomal Microtubule Assembly.

Figure 5

Spindle formation around sperm chromosomes was monitored in control extract or in ΔIncenp extract complemented with either Aurora B/IncenpΔ1-327 in the presence or absence of anti-Incenp antibody (2.5, 5, or 10 ng/μl), or Aurora B/Incenp/Dasra A. Extracts were prepared as described (Figure 2), except that 24 μg/ml cyclin BΔ90 was added. A full analysis of each condition is described in Supplemental Figure S8. (A) Representative images of structures formed at 30, 45 and 75 minutes after entry into M phase for ΔIncenp extracts containing Aurora B/IncenpΔ1-327, Aurora B/IncenpΔ1-327 + 2.5 ng/μl anti-Incenp, or Aurora B/IncenpΔ1-327 + 10 ng/μl anti-Incenp. Representative bipolar spindle structures formed (inset) are shown for the 30 and 45-minute time points. Rhodamine-tubulin (red) and Hoechst 33258 (green) were used to visualize microtubules and DNA, respectively. Bars, 20 μm. (B) All samples were analyzed by quantitative Western blot at 30 and 60 minutes after entry into M phase. Left-most lanes represent indicated amounts of control extracts.