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. Author manuscript; available in PMC: 2008 Mar 15.
Published in final edited form as: Mol Cell Endocrinol. 2006 Dec 16;267(1-2):17–25. doi: 10.1016/j.mce.2006.11.011

Fig. 2.

Fig. 2

Sucrose density gradient analysis of raft association of PC3. N2A cells transfected with PC3 constructs were extracted with 1% TX-100/TNE and fractionated on a sucrose gradient. Fractions were collected from the top of the gradient (1–10) and precipitated proteins analyzed by Western blotting using various antibodies. Left column: PC3 constructs used in the transfection. Right column: antibodies used for Western blots. The blots of the gradient for PC3-616 and PC3-ΔTM were stripped and reprobed with antibodies to caveolin-1 or the transferrin receptor (TfR) as controls.