Steady state secretion of full length and truncated PC3 from transfected N2A cells.
(A) Representative Western blot of PC3 in concentrated basal medium (20 μl) collected from 3 consecutive 1 hour incubations (1st hour, 2nd hour and 3rd hour) using the PC3-fus antibody and analyzed with the Odyssey Infrared Imager. Basal medium was collected from cells transfected with empty vector (V), or vector encoding full-length PC3 (FL), C-terminus truncated PC3-638 (638) and PC3-616 (616). (B) Quantification of PC3 levels detected on Western blots from 4 separate experiments. PC3 levels for PC3-638 and PC3-616 constructs are shown as a percentage of full-length PC3 (PC3-FL) at each time point. Note that higher levels of PC3-616 in the medium compared to full-length PC3 and PC3-638.