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. Author manuscript; available in PMC: 2008 Mar 15.
Published in final edited form as: Mol Cell Endocrinol. 2006 Dec 16;267(1-2):17–25. doi: 10.1016/j.mce.2006.11.011

Fig. 5.

Fig. 5

Stimulated secretion of PC3 from transfected N2A cells.

(A) Representative PC3 Western blot of concentrated basal (B) or stimulated (S) media collected from three PC3 constructs. Medium was collected from cells transfected with empty vector (V), or vectors encoding full-length PC3 (FL), and C-terminally truncated PC3-638 (638) and PC3-616 (616). There were significant increases in the stimulated secretion of PC3 in FL and 638 transfected cells, but not for PC3-616.

(B) Quantification of PC3 secretion levels expressed as mean ±SEM from stimulated media, (hashed bars) compared to basal media (open bars) from Western blots (N=5). AU: arbitrary units; (C) Analysis of PC3 activity using the fluorescent substrate Boc-Arg-Val-Arg-Arg-MCA for the enzymatic activity in basal release (clear bars) or stimulated release medium (hatched bars). Levels of enzymatic activity were calculated as total pmol AMC released. Data are presented as mean release ±SEM from two experiments in quadruplicates after subtraction of AMC from the vector-only controls. The enzymatic activity in vector-only transfected cells in basal medium was 1.03 ± 0.02 pmol AMC and in stimulation medium 1.16± 0.03 pmol AMC.