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. Author manuscript; available in PMC: 2007 Nov 27.
Published in final edited form as: FEBS Lett. 2006 Nov 3;580(27):6405–6412. doi: 10.1016/j.febslet.2006.10.054

Figure 2.

Figure 2

RKIP has no effect on the phosphorylation of Raf-1 by v-SRC or PAK in vitro. Purified recombinant GST-Raf-1(325-349) and GST-Raf-1(325-349SS338/339AA) peptides were tested for phosphorylation by Myc-Pak (T423E) or HA-v-Src in the presence of increasing amounts of RKIP (1, 3 and 9 μM) or BSA (9 μM) as control. Phosphorylated proteins were detected by autoradiography. PAK and v-SRC proteins were detected by Western blotting.