Table 1.
Kinetic analysis of RKIP binding to immobilized Raf-1 peptides by surface plasmon resonance. Binding was performed at 25°C as described in Materials and Methods. Shown are the affinity (KD), association (ka), and dissociation constants (kd) with their standard errors (SE) and chi2 values; RU, resonance units.
| Peptide | ka (1/Ms) | SE(ka) | kd (1/s) | SE(kd) | RUmax | SE (Rmax) | KD | chi2 |
|---|---|---|---|---|---|---|---|---|
| NR1 SSYY | 46.1 | 3.98 | 0.168 | 2.56×10-3 | 3.2×10-3 | 258 | 3.62×10-3 | 0.281 |
| NR2 pSpSYY | 16.8 | 1.52 | 9.71×10-3 | 2.82×10-5 | 242 | 16.8 | 5.77×10-4 | 0.873 |
| NR3 SSpYpY | 23.5 | 2.87 | 0.0127 | 2.65×10-4 | 206 | 21.6 | 5.42×10-4 | 0.187 |
| NR4 pSpSpYpY | 321 | 4.33 | 3.25 ×10-3 | 2.00×10-5 | 302 | 3.48 | 1.01×10-5 | 0.397 |
| NR5 pSpSYpY | 2218 | 54 | 0.04518 | 3.3 ×10-4 | 105.6 | 2.0 | 2.04×10-5 | 0.366 |