Table 2.
Mutations in genomic segments of attenuated MOP/LAS reassortant (clone ML29)
| Nucleotide | L RNA position | ORF/NCR | Nucleotide | S RNA position | ORF/NCR |
|---|---|---|---|---|---|
| A/C | 6 | 5′ | A/G | 869 | GPC |
| U/G | 8 | 5′ | U/C | 1366 | GPC |
| C/U | 810 | RdRp (L) | G/A | 1842 | NP |
| G/A | 1423 | RdRp (L) | G/U | 1849 | NP |
| U/C | 3319 | RdRp (L) | U/C | 2785 | NP |
| U/C | 3519 | RdRp (L) | G/A | 3328 | 3′ |
| U/C | 3664 | RdRp (L) | |||
| G/A | 4002 | RdRp (L) | |||
| U/C | 4177 | RdRp (L) | |||
| A/U | 4665 | RdRp (L) | |||
| A/C | 7264 | 3′ | |||
| U/G | 7266 | 3′ | |||
| Amino acid | Position | RdRp | Amino acid | Position | GPC/NP |
| Y/N | 851 | RdRp (L) | K/E | 272 | GPC (GP2) |
| R/G | 1233 | RdRp (L) | A/D | 485 | NP |
| D/N | 2136 | RdRp (L) | N/S | 173 | NP |
ORF, open reading frame; NCR, 5′ and 3′ non-coding regions; RdRp, RNA-dependent RNA polymerase (L protein); GPC, glycoprotein precursor; NP, nucleocapsid protein. Non-conservative amino acid substitutions are underlined