Table 4.
Fluorescence intensity of each state relative to the acid unfolded state estimated by modeling the folding kinetics of WT* and the single-Trp variants, based on scheme 1.
| Fluorescence intensity relative to the acid unfolded state
|
||||||||
|---|---|---|---|---|---|---|---|---|
| State | WT* SNase | Trp15 SNase | Trp27 SNase | Trp61 SNase | Trp76 SNase | Trp91 SNase | Trp102 SNase | Trp121 SNase |
| U | 1.47 | 1.24 | 1.32 | 1.32 | 1.21 | 1.26 | 1.48 | 1.26 |
| I1 | 1.42 | 1.35 | 1.27 | 1.44 | 1.75 | 1.29 | 1.77 | 1.63 |
| I2 | 1.5 | 1.43 | 0.6 | 1.65 | 1.8 | 1.5 | 1.77 | 1.63 |
| M | 3.47 | 0.65 | 0.69 | 1.1 | 3.5 | 3.15 | 4.15 | 1.93 |
| N | 3.47 | 0.65 | 0.69 | 1.1 | 3.53 | 3.15 | 4.15 | 1.93 |
| U′ | 1.43 | 1.24 | 1.32 | 1.32 | 1.21 | 1.26 | 1.48 | 1.26 |
| I′ | 1.43 | 1.35 | 1.27 | 1.44 | 1.75 | 1.29 | 1.77 | 1.63 |