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. 2007 Jun 5;2007:29632. doi: 10.1155/2007/29632

Figure 1.

Figure 1

Figure 1

Figure 1

Rosiglitazone stimulates the activation of TSC2. (a) Time-dependent effect of rosiglitazone on TSC2 phosphorylation. Cellular protein was isolated from H2106 cells that were cultured with increasing concentrations of rosiglitazone (Rosig.) for 1 hour followed by Western blot analysis with antibodies against total TSC2 and phosphorylated TSC2 (p-TSC2). (b) Effect of PPARγ antagonists on rosiglitazone-induced TSC2 phosphorylation. Cellular protein was isolated from H2106 cells cultured for up to 2 hours in the presence or absence of GW9662 (20 μM) before exposure of cells to rosiglitazone (Rosig., 10 μM) for an additional 24 hours, then subjected to Western blot analysis for total TSC2 and phosphorylated TSC2 (p-TSC2). (c) Effect of PPARγ siRNA on rosiglitazone-induced TSC2 phosphorylation. H2106 cells were transfected with control or PPARγ siRNA (100 nM each) for 48 hours before exposing the cells to rosiglitazone (Rosig., 10 μM) for up to 24 hours. Afterwards, we performed Western blot analysis for total TSC2 and phosphorylated TSC2 (p-TSC2). Actin served as internal control for normalization purposes (Con, indicates untreated control cells).