Figure 3.

Silencing TSC2 restored the activity of p70S6K. (a) TSC2 siRNA blocks TSC2 production. H2106 cells were transfected with control or TSC2 siRNA (100 nM each) for 30 hours. Afterwards, we performed Western blot analysis for TSC2 proteins (Con, indicates untreated cells). (b) TSC2 siRNA ameliorates the inhibitory effect of rosiglitazone on p70S6K phosphorylation. H2106 cells were transfected with control or TSC2 siRNA (100 nM each) for 48 hours before exposing the cells to rosiglitazone (Rosig., 10 μM) for up to 24 hours. Afterwards, we performed Western blot analysis for p70S6K proteins (Con, indicates untreated cells). The representative data shown here is obtained from at least three separate experiments. Graphs are densitometry results. (*indicates significant differences as compared to the zero hour or untreated cells (P < .05); **indicates significance of combination treatment as compared with rosiglitazone alone (P < .05).)