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. Author manuscript; available in PMC: 2008 Mar 15.
Published in final edited form as: Neuron. 2007 Mar 15;53(6):789–803. doi: 10.1016/j.neuron.2007.02.018

Figure 5.

Figure 5

Electroporation using regular salt-form Ca2+ dyes. A, In vivo two-photon imaging of the barrel-cortex neurons labeled by two separate electroporations using 5% CG-1 hexapotasssium and 5% X-rhod-1 tripotassium, respectively. The loading parameters were both -3 μA current amplitude, 25 ms pulse duration and 1200 pulses delivered at 2 Hz. A1 shows the z-projection of labeled dendrites imaged at a depth range of 50 to 115 μm below the pia. A2 is the z-projection of images between 185 and 250 μm, showing the labeled cell bodies. Scale bar, 50 μm. B, Ca2+ responses of a neuron labeled with CG-1 hexapotassium salt. The two traces show the responses to one (upper) and five (lower) electric stimuli as indicated by red dots. For each trace, five imaging trials were averaged.